OSM Protein (Native, Human)

Product Specifications

Abbreviation

OSM

Synonyms

MGC20461

Host

HEK 293

Tag

Native

Endotoxin

<5 EU per mg protein

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is typically 0.3-1.2 ng/mL.The specific activity of recombinant human OSM is >30,000 Units/μg, which is calibrated against the human OSM WHO International Standard (NIBSC code: 93/564) .

Function

Met1-Arg221

Molecular Weight

The recombinant human OSM consists of 196 amino acids and predicts a molecular mass of 22.16 kDa. As a result of glycosylation, it migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.