NRG1 Protein (N-cleavage, Human)

1.Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 2-10 ng/mL. 2.Western blot analysis of extracts from serum-starved MCF-7, untreated (-) or treated with neuregulin-1 (Cat # 11609-HNCH) (100 ng/mL, 5 min) using Phospho-HER3/ErbB3 (Tyr1289) rabbit monoclonal Antibody (Cat# 111146-R0004) at 1:2000 dilution (upper), or Anti-HER3/ERBB3 Antibody, Rabbit Polyclonal (Cat# 10201-RP02) at 1:2000 dilution (middle), or Beta-Tubulin Loading Control Antibody, Mouse MAb (Cat# 100109-MM05T) at 1:2000 dilution (lower) (Routinely tested) .

The recombinant human NRG1 comprises 245 amino acids and has a predicted molecular mass of 26.8 kDa. As a result of glycosylation, it migrates as an approximately 47.7 kDa band in SDS-PAGE under reducing conditions.