NECTIN4 Protein (C-His, Human)

Product Specifications

Abbreviation

NECTIN4

Synonyms

EDSS1, LNIR, nectin-4, PRR4, PVRL4

Host

HEK 293

Tag

C-His

Endotoxin

< 0.5 EU per mg protein.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human NECTIN4 / PVRL4 Protein (His Tag) (Cat: 19771-H08H-UE) at 1 μg/mL (100 μL/well) can bind Anti-Nectin4 (Research Grade Enfortu * mab Biosimilar), the EC50 is 0.6-2.0 ng/mL (Routinely tested) . 2.Anti-Nectin4 (Research Grade Enfortumab Biosimilar) captured on Protein A chip, can bind Recombinant Human NECTIN4 / PVRL4 Protein (His Tag) (Cat: 19771-H08H-UE) with an affinity constant of 8.600 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Function

Met1-Ser349

Molecular Weight

The recombinant human NECTIN4 consists of 329 amino acids and predicts a molecular mass of 35.56 kDa. It migrates as approximately 40-48 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 40.5 kDa verified by SEC-MALS (Routinely tested) .

Species

Human