NECTIN4 Protein (C-His, Human)

1.Measured by its binding ability in a functional ELISA. Immobilized Human NECTIN4 His (Cat: 19771-H08H) at 2 μg/ml (100 μl/well) can bind Human PVRL1 His (Cat: 11611-H08H), biotinylated, the EC50 of Human PVRL1 His, biotinylated is 200-800 ng/mL (Routinely tested) . 2.Immobilized Recombinant Human NECTIN4 / PVRL4 Protein (His Tag) (Cat: 19771-H08H) at 1 μg/mL (100 μL/well) can bind Anti-Nectin4 (Research Grade Enfortumab Biosimilar), the EC50 is 0.6-2.5 ng/mL (QC tested) . 3.Loaded Recombinant Human Nectin-1/PVRL1 Protein, hFc Tag (Cat. No. 11611-H02H) on ProA Biosensor, can bind Recombinant Human Nectin-4 Protein, His Tag (Cat. No. 19771-H08H) with an affinity constant of 0.32 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Anti-Nectin4 (Research Grade Enfortumab Biosimilar) captured on Protein A chip can bind Recombinant Human Nectin-4 Protein, His Tag (Cat. No. 19771-H08H) with an affinity constant of 7.66 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .