NECTIN2 Protein (C-human IgG1-Fc, Human)

1. Measured by its binding ability in a functional ELISA. Immobilized human CD226 at 2 μg/ml (100 μl/well) can bind human CD112 with a linear range of 6.4-800 ng/ml. 2. Immobilized PVRIG Protein, Cynomolgus, Rhesus, Recombinant (His Tag) (Cat:90971-C08H) at 2 μg/ml (100 μl/well) can bind Nectin-2 Protein, Human, Recombinant (hFc Tag) (Cat:10005-H02H), the EC50 of Nectin-2 Protein, Human, Recombinant (hFc Tag) is 0.2-1.2 ng/mL. 3.Loaded Human PVRIG Protein, mFc Tag (Cat.No.28312-H38H2) on AMC Biosensor, can bind Human Nectin-2 protein, hFc Tag (Cat.No.10005-H02H) with an affinity constant of 8.784nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested) .

The recombinant mature human CD112/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 567 amino acids and has a calculated molecular mass of 62.3 kDa. As a result of glycosylation, the rh CD112/Fc monomer migrates as an approximately 70-80 kDa protein in SDS-PAGE under reducing conditions.