LAG3 Protein (C-His, Human)

1.Immobilized Recombinant Human LAG3/CD223 Protein (ECD, His Tag) (Cat: 16498-H08H-UE) at 2 μg/mL (100 μL/well) can bind Anti-LAG3 (Research Grade Relatlimab Biosimilar), the EC50 is 5-15 ng/mL (Routinely tested) . 2.Loaded Anti-LAG3 (Research Grade Relatlimab Biosimilar) on ProA Biosensor, can bind Recombinant Human LAG3/CD223 Protein (ECD, His Tag) (Cat. No. 16498-H08H-UE) with an affinity constant of 0.104 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Relatlimab-Derived Anti-LAG3 Antibody, Human IgG4 captured on Protein A chip, can bind Recombinant Human LAG3/CD223 Protein (ECD, His Tag) (Cat: 16498-H08H-UE) with an affinity constant of 0.342 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The recombinant human LAG3 consists of 439 amino acids and predicts a molecular mass of 47.6 kDa. As a result of glycosylation, it migrates as an approximately 55-60 KDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 73.9 kDa verified by SEC-MALS (Routinely tested) .