LAG3 Protein (C-His, Human)

1.Immobilized Recombinant Human LAG3/CD223 Protein (ECD, His Tag) (Cat: 16498-H08H) at 2 μg/mL (100 μl/well) can bind Anti-LAG3 (Research Grade Relatli*mab Biosimilar), the EC50 is 7-21ng/mL (QC tested) . 2.Captured Anti-LAG3 Mab (Human IgG4) on proA Chip can bind LAG3 (Cat.No.16498-H08H) with an affinity constant of 88.45 pM as determined in a SPR assay (Biacore T200) (Routinely tested) . 3.Loaded Anti-LAG-3 Mab (Human IgG4) on ProA Biosensor, can bind human LAG-3 protein, His Tag (Cat.No.16498-H08H) with an affinity constant of 0.617nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested) .

The recombinant human LAG3 consists of 439 amino acids and predicts a molecular mass of 47.6 kDa. As a result of glycosylation, it migrates as an approximately 55-60 KDa band in SDS-PAGE under reducing conditions.