IL6 Protein (Native, Human)
Product Specifications
Abbreviation
IL6
Synonyms
BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6
Host
HEK 293
Tag
Native
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE.
Bioactivity
1.Immobilized Recombinant Human IL-6R / CD126 Protein (ECD, Fc Tag) (Cat: 10398-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-6 protein, Biotinylated (Cat: 10395-HNAH-B), the EC50 is 30-90 ng/mL (QC tested) . 2.Loaded Biotinylated Recombinant Human IL-6 protein (Cat. No. 10395-HNAH-B) on SA Biosensor, can bind Recombinant Human IL-6R Protein, His Tag (Cat. No. 10398-H08H) with an affinity constant of 31.750 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Met1-Met212
Molecular Weight
The recombinant Human IL6 consists of 183 amino acids and predicts a molecular mass of 20.82 kDa. It migrates as an approximately 27.5 kDa band in SDS-PAGE under reducing conditions.
Species
Human
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