IL5RA Protein (C-His, Human)

Product Specifications

Abbreviation

IL5RA

Synonyms

CD125, CDw125, HSIL5R3, IL5R

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human IL-5 Protein (Fc Tag) (Cat: 15673-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-5R alpha Protein (Membrane-bound, His Tag) (Cat: 10392-H08H1), the EC50 is 60-220 ng/mL. 2.Loaded Recombinant Human IL-5R alpha Protein, Membrane-bound, His Tag (Cat. No. 10392-H08H1) on HIS1K Biosensor, can bind Recombinant Human IL-5 Protein (Cat. No. 15673-HNAH) with an affinity constant of 43.200 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Biotinylated Recombinant Human IL-5 Protein, His & AVI Tag (Cat. No. 15673-H49H-B) on SA Biosensor, can bind Recombinant Human IL-5R alpha Protein, Membrane-bound, His Tag (Cat. No. 10392-H08H1) with an affinity constant of 17.800 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested) .

Function

Met1-Trp342

Molecular Weight

The recombinant Human IL5RA comprises 333 amino acids and has a predicted molecular mass of 38.23 kDa. It migrates as approximately 48.1 kDa band in SDS-PAGE under reducing conditions.

Species

Human