IL3Ra Protein (C-His, Human)

1.Loaded Human IL-3R alpha Protein, His Tag (Cat. No. 10518-H08H) on His1K Biosensor, can bind Human IL3 protein (Cat. No. 11858-HNAE) with an affinity constant of 0.183μM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested) . 2.Recombinant Human IL3RA / CD123 Protein (His Tag) (Cat: 10518-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human IL3 / IL-3 Protein (Cat: 11858-HNAE) with an affinity constant of 0.257 μM as determined in an SPR assay (Biacore 8K) (QC tested) .

The secreted recombinant human IL3RA comprises 298 amino acids and has a predicted molecular mass of 34.5 kDa. As a result of glycosylation, the apparent molecular mass of rh IL3RA is approximately 50-60 kDa in SDS-PAGE under reducing conditions.