IL3Ra Protein (C-His, Human)
Product Specifications
Abbreviation
IL3Ra
Synonyms
CD123, hIL-3Ra, IL-3R-alpha, IL3R, IL3RA, IL3RAY, IL3RX, IL3RY
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Loaded Human IL-3R alpha Protein, His Tag (Cat. No. 10518-H08H) on His1K Biosensor, can bind Human IL3 protein (Cat. No. 11858-HNAE) with an affinity constant of 0.183μM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested) . 2.Recombinant Human IL3RA / CD123 Protein (His Tag) (Cat: 10518-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human IL3 / IL-3 Protein (Cat: 11858-HNAE) with an affinity constant of 0.257 μM as determined in an SPR assay (Biacore 8K) (QC tested) .
Function
Met1-Trp304
Molecular Weight
The secreted recombinant human IL3RA comprises 298 amino acids and has a predicted molecular mass of 34.5 kDa. As a result of glycosylation, the apparent molecular mass of rh IL3RA is approximately 50-60 kDa in SDS-PAGE under reducing conditions.
Species
Human
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