IL3 Protein (Native, Human)

Product Specifications

Abbreviation

IL3

Synonyms

IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF

Host

E. coli

Tag

Native

Endotoxin

< 5 EU per mg of the protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.15-0.75 ng/ml. 2.Loaded Human IL-3R alpha Protein, His Tag (Cat. No. 10518-H08H) on His1K Biosensor, can bind Human IL3 protein (Cat. No. 11858-HNAE) with an affinity constant of 0.183μM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested) . 3.Recombinant Cynomolgus IL-3R alpha/CD123 Protein, His Tag (Cat. No. 90998-C08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human IL-3 Protein (Cat. No. 11858-HNAE) with an affinity constant of 0.363 μM as determined in an SPR assay (Biacore T200) (QC tested) .