IL2RA Protein (C-His, Human)

1.Measured by its ability to inhibit IL2-induced proliferation of M07e cells. The ED50 for this effect is 0.2-1.2 μg/mL in the presence of 5 ng/mL of recombinant human IL2 (Routinely tested) . 2.Loaded Recombinant Human CD25/IL-2R alpha Protein, His Tag (Cat. No. 10165-H08H) on His1K Biosensor, can bind Recombinant Human IL-2 Protein, no Tag (Cat. No. 11848-HNAE2) with an affinity constant of 44.8 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 3.Recombinant Human IL2Ra / CD25 Protein (His Tag) (Cat: 10165-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human IL2 Protein, Low Endotoxin (Cat: 11848-HNAH1-E) with an affinity constant of 22.270 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant human IL2Rα consists of 203 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL2Rα is approximately 44 kDa due to glycosylation.