IL1R1 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

IL1R1

Synonyms

CD121A, CRMO3, D2S1473, IL-1R-alpha, IL-1RT1, IL1R, IL1RA, IL1RT1, P80

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its ability to bind human IL1B in a functional ELISA. 2.Recombinant Human IL1R1 Protein, hFc Tag (Cat. No. 10126-H02H) captured on Protein A chip, can bind Recombinant Human IL-1 beta Protein (Cat. No. 10139-HNAE) with an affinity constant of 0.942 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Met1-Thr332

Molecular Weight

The recombinant human IL1R1/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 553 amino acids after removal of the signal peptide and has a predicted molecular mass of 63 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 80-90 kDa due to glycosylation.