IL1B Protein (Native, Human)
Product Specifications
Abbreviation
IL1B
Synonyms
IL-1, IL-1B, IL1-BETA, IL1beta, IL1F2
Host
E. coli
Tag
Native
Endotoxin
< 5 EU per mg of the protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.2-2 ng/mL. 2.Recombinant Human IL1R1 Protein, hFc Tag (Cat. No. 10126-H02H) captured on Protein A chip, can bind Recombinant Human IL-1 beta Protein (Cat. No. 10139-HNAE) with an affinity constant of 0.942 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Recombinant Human IL1R2 Protein, hFc Tag (Cat. No. 10111-H02H) captured on Protein A chip, can bind Recombinant Human IL-1 beta Protein (Cat. No. 10139-HNAE) with an affinity constant of 2.008 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .
Function
Ala117-Ser269
Molecular Weight
The recombinant human IL1β consisting of 154 amino acids migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.
Species
Human
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