IL1B Protein (Native, Human)

1.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.2-2 ng/mL. 2.Recombinant Human IL1R1 Protein, hFc Tag (Cat. No. 10126-H02H) captured on Protein A chip, can bind Recombinant Human IL-1 beta Protein (Cat. No. 10139-HNAE) with an affinity constant of 0.942 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Recombinant Human IL1R2 Protein, hFc Tag (Cat. No. 10111-H02H) captured on Protein A chip, can bind Recombinant Human IL-1 beta Protein (Cat. No. 10139-HNAE) with an affinity constant of 2.008 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant human IL1β consisting of 154 amino acids migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.