IL13 Protein (N-human IgG1-Fc, Human)

1.Measured by its binding ability in a functional ELISA.Immobilized recombinant human IL13RA2 at 8 μg/ml (100 μl/well) can bind IL13 with a linear range of 0.25-8.0 ng/ml. 2.Loaded Recombinant Human IL-13 Protein, hFc Tag (Cat. No. 10369-H01H) on ProA Biosensor, can bind Recombinant Human IL-13 R alpha 2/IL13RA2 Protein, His Tag (Cat. No. 10350-H08H) with an affinity constant of 1.99 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human Fc/IL13 chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 349 amino acids and predicts a molecular mass of 39 kDa. The apparent molecular mass of rhIL13/Fc monomer is approximately 50-55 kDa in SDS-PAGE under reducing conditions due to glycosylation.