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IL12B & IL23A Protein (C-His, Human)

Product Specifications

Abbreviation

IL12B & IL23A

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Bioactivity

1.Immobilized Recombinant Human IL-23 (IL12B&IL23A Heterodimer) Protein (His Tag), Biotinylated (Cat: CT048-H08H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) (Cat: 13840-H02H), the EC50 is 10-30 ng/mL (QC tested) . 2.Immobilized Recombinant Human IL-23 (IL12B&IL23A Heterodimer) Protein (His Tag), Biotinylated (Cat: CT048-H08H-B) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 200-600 ng/mL (Routinely tested) . 3.Immobilized Anti-IL-12 & IL-23 (P40) (Research Grade Ustekin*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-23 (IL12B & IL23A Heterodimer) Protein (His Tag) (Cat: CT048-H08H-B), the EC50 is 3-10 ng/mL (Routinely tested) . 4.Immobilized Anti-IL-23 Antibody (P19) (Research Grade Risankiz*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-23 (IL12B & IL23A Heterodimer) Protein (His Tag) (Cat: CT048-H08H-B), the EC50 is 3-10 ng/mL (Routinely tested) . 5.Loaded Biotinylated Recombinant Human IL-23 Protein, His Tag (Cat. No. CT048-H08H-B) on SA Biosensor, can bind Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) with an affinity constant of 55.5 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 6.Loaded Anti-IL-12 & IL-23 (P40) (Research Grade Ustekin*mab Biosimilar) on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag, Amine-Labeled Biotinylated (Cat. No. CT048-H08H-B) with an affinity constant of 1.13nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 7.Loaded Anti-IL-23 Antibody (P19) (Research Grade Risankiz*mab Biosimilar) on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag, Amine-Labeled Biotinylated (Cat. No. CT048-H08H-B) with an affinity constant of 2.13nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 8.Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) captured on Protein A chip can bind Amine-Labeled Biotinylated Recombinant Human IL-23 Protein, His Tag (Cat. No. CT048-H08H-B) with an affinity constant of 1.260 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Function

Met1-Ser328 & Arg20-Pro189

Molecular Weight

The recombinant human IL23 complex (IL23A&IL12B) consists 502 amino acids and predicts a molecular mass of 55.8 kDa.

Species

Human

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