IL12B & IL23A Protein (C-His, Human)

1.Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.1-1ng/mL. 2.Immobilized Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) (Cat: 13840-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-23 (IL12B & IL23A Heterodimer) Protein (His Tag) (Cat: CT048-H08H), the EC50 is 15-60 ng/mL (QC tested) . 3.Immobilized Recombinant Human IL-23 (IL12B & IL23A Heterodimer) Protein (His Tag) (Cat: CT048-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 25-80 ng/mL (Routinely tested) . 4.Immobilized Anti-IL-12 & IL-23 (P40) (Research Grade Ustekin*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-23 (IL12B & IL23A Heterodimer) Protein (His Tag) (Cat: CT048-H08H), the EC50 is 6-18 ng/mL (Routinely tested) . 5.Immobilized Anti-IL-23 Antibody (P19) (Research Grade Risankiz*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-23 (IL12B & IL23A Heterodimer) Protein (His Tag) (Cat: CT048-H08H), the EC50 is 10-30 ng/mL (Routinely tested) . 6.Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) captured on Protein A chip can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT048-H08H) with an affinity constant of 0.6961 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 7.Loaded Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT048-H08H) with an affinity constant of 1.25 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 8.Loaded Anti-IL-23 Antibody (P19) (Research Grade Risankiz*mab Biosimilar) on ProA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT048-H08H) with an affinity constant of 0.075 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 9.Loaded Anti-IL-12 & IL-23 (P40) (Research Grade Ustekin*mab Biosimilar) on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT048-H08H) with an affinity constant of 0.568 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 10.Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) captured on Protein A chip can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT048-H08H) with an affinity constant of 0.550 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The recombinant human IL23 (IL23A&IL12B) consists of 502 amino acids and predicts a molecular mass of 55.8 kDa. The apparent molecular mass of human IL23 heterodimer is approximately 58.3 kDa respectively in SDS-PAGE under reducing conditions.