IL12B & IL23A Protein (C-His, Rat)

Product Specifications

Abbreviation

IL12B & IL23A

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

1.Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-3ng/mL. 2.Loaded Recombinant Rat IL-23 Receptor Protein, hFc Tag (Cat. No. 80395-R02H) on proA Biosensor, can bind Recombinant Rat IL-23 Protein, His Tag (Cat. No. CT045-R08H) with an affinity constant of 32.700 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested) .

Function

Met1-Ala196 & Met1-Ser335

Molecular Weight

The recombinant rat IL23A & the rat IL12B comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.2 (21+37.2) KDa. The apparent molecular mass of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE.