IL12B & IL23A Protein (C-His, Mouse)

1. Immobilized Human IL23 Receptor hFc (Cat:13840-H02H) at 2 μg/ml (100 μl/well) can bind Mouse IL-23 His (Cat:CT028-M08H), the EC50 of Mouse IL-23 His is 50-250 ng/mL. 2. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-1 ng/mL. 3.Loaded Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) on proA Biosensor, can bind Recombinant Mouse IL-23 Protein, His Tag (Cat. No. CT028-M08H) with an affinity constant of 6.13 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Loaded Recombinant Rat IL-23 Receptor Protein, hFc Tag (Cat. No. 80395-R02H) on proA Biosensor, can bind Recombinant Mouse IL-23 Protein, His Tag (Cat. No. CT028-M08H) with an affinity constant of 93.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant mouse IL23 (mouse IL23A&IL12B Heterodimer) comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.3 (21.1+ 37.2) KDa. The apparent molecular mass of the recombinant protein is approximately 47,45 and 26 KDa respectively in SDS-PAGE under reducing conditions.