HHLA2 Protein (C-His, Human)
Product Specifications
Abbreviation
HHLA2
Synonyms
B7-H5, B7-H7, B7H7, B7y
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Bioactivity
1.Immobilized HHLA2 Protein, Human, Recombinant (His Tag) (Cat:16139-H08H) at 2 μg/mL (100 μL/well) can bind CD28H/TMIGD2 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:13820-H02H), the EC50 is 140-420 ng/mL. 2.Loaded Recombinant Human B7-H7 / HHLA2 Protein, His Tag (Cat. No. 16139-H08H) on His1K Biosensor, can bind Recombinant Human KIR3DL3 Protein, hFc Tag (Cat. No. 16055-H02H) with an affinity constant of 18.5 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Met1-Asn344
Molecular Weight
The recombinant human HHLA2 consists of 333 amino acids and predicts a molecular mass of 38.4 kDa. As a result of glycosylation, it migrates as an approximately 55 KDa band in SDS-PAGE under reducing conditions.
Species
Human
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