HHLA2 Protein (C-His, Human)

1.Immobilized HHLA2 Protein, Human, Recombinant (His Tag) (Cat:16139-H08H) at 2 μg/mL (100 μL/well) can bind CD28H/TMIGD2 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:13820-H02H), the EC50 is 140-420 ng/mL. 2.Loaded Recombinant Human B7-H7 / HHLA2 Protein, His Tag (Cat. No. 16139-H08H) on His1K Biosensor, can bind Recombinant Human KIR3DL3 Protein, hFc Tag (Cat. No. 16055-H02H) with an affinity constant of 18.5 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human HHLA2 consists of 333 amino acids and predicts a molecular mass of 38.4 kDa. As a result of glycosylation, it migrates as an approximately 55 KDa band in SDS-PAGE under reducing conditions.