HA Protein (C-His, H5N1)

Product Specifications

Abbreviation

Host

Baculovirus-insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 85 % as determined by SDS-PAGE

Bioactivity

1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to agglutinate guinea pig red blood cells. 3.Immobilized Recombinant H5N1 (A/chicken/VietNam/NCVD-016/2008) HA Protein (His Tag) (Cat: 40158-V08B) at 2 μg/ml (100 μl/well) can bind a broadly neutralizing influenza HA stem-specific antibody, the EC50 is 10-40 ng/mL.

Function

Met1-Gln532

Molecular Weight

The recombinant hemagglutinin of Influenza A virus (A/chicken/VietNam/NCVD-016/2008 (H5N1) ) comprises 527 amino acids and has a predicted molecular mass of 60.2 kDa. The apparent molecular mass of the protein is approximately 66.2 kDa in SDS-PAGE under reducing conditions.