GPRC5D Protein (N-Flag & C-His, Human)

Product Specifications

Abbreviation

GPRC5D

Host

HEK 293

Tag

N-Flag & C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 75 % as determined by SDS-PAGE.

Bioactivity

1.Immobilized Recombinant Human GPRC5D-Nanodisc Protein (Cat:24447-H18H-NA) at 5 ug/mL (100 μL/well) can bind Anti-GPRC5D Monoclonal Antibody, Human lgG1, the EC50 is 2-30 ng/mL. 2.Loaded Anti-GPRC5D Antibody on Protein A Biosensor, can bind Recombinant Human GPRC5D-Nanodisc Protein, His & FLAG Tag (Cat: 24447-H18H-NA) with an affinity constant of 0.782 nM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 3.Captured Anti-GPRC5D antibody on CM5 chip via anti-Human IgG Fc, can bind Recombinant Human GPRC5D-Nanodisc Protein, His & FLAG Tag (Cat: 24447-H18H-NA) with an affinity constant of 9.609 nM as determined in a SPR assay (Biacore T200) (Routinely tested)