FLT1 Protein (C-His, Human)

1.Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) (Conn, G. et al.,1990 , Proc. Natl. Acad. Sci. USA 87:1323.) . The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL human VEGF165. 2.Immobilized Recombinant Human VEGFR1 / FLT-1 Protein (His Tag) (Cat: 10136-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein (Cat: 11066-HNAH), the EC50 is 3.8-11.6 ng/mL.

The secreted recombinant human VEGFR1 consists of 741 amino acids with the predicted molecular mass of 83.7 kDa. As a result of glycosylation, rhVEGFR1 migrates as an approximately 110-120 kDa band in SDS-PAGE under reducing conditions.