FCGRT & B2M Protein (C-His, Mouse)

1.Loaded Mouse FcRn recombinant protein (Cat. CT029-M08H) on His1k Biosensor, can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) with an affinity constant of 8.3 nM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 2.Captured Mouse FcRn recombinant protein (Cat. CT029-M08H) on Anti-His Chip can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) with an affinity constant of 32.9 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Captured Mouse FcRn recombinant protein (Cat. CT029-M08H) on Anti-His Chip can bind Anti-PD1 (Research Grade Nivolumab Biosimilar) with an affinity constant of 76.8 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 4.Loaded Recombinant Mouse FCGRT & B2M Heterodimer Protein, His Tag (Cat. No. CT029-M08H) on His1K Biosensor, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) with an affinity constant of 4.98nM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 5.Recombinant Mouse FCGRT & B2M Heterodimer Protein (Cat: CT029-M08H) captured on NTA chip, can bind anti-ERBB2 (Research grade trastuz*mab biosimilar) with an affinity constant of 0.844 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The recombinant heterodimer of mouse FCGRT / B2M comprises 385 (285 + 100) amino acids and has a calculated molecular mass of 43.5 (31.8 + 11.7) kDa. The apparent molecular mass of FCGRT / B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions The molecular weight of this protein is around 50-60 kDa verified by SEC-MALS (Routinely tested) .