FCGR4 Protein (C-AVI & His, Mouse)

1.Loaded Recombinant Mouse FCGR4 Protein, His Tag, Biotinylated (Cat. No. 50036-M27H-B) on SA Biosensor, can bind Anti-CD3 (Research Grade OKT3 Biosimilar) with an affinity constant of 22.6nM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 2.Loaded Biotinylated Recombinant Mouse CD16-2/FCGR4/Fc gamma RIV Protein, His & AVI Tag (Cat. No. 50036-M27H-B) on SA Biosensor, can bind Anti-CD20 (Research Grade Rituximab Biosimilar) with an affinity constant of 0.475 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Anti-CD3 (OKT3) (Research Grade Muromonab Biosimilar) at 5 μg/mL (100 μL/well) can bind Recombinant Mouse CD16-2/FCGR4/Fc gamma RIV Protein (His & AVI Tag), Biotinylated (Cat: 50036-M27H-B) the EC50 is 3.1 ng/mL (Routinely tested) .

The recombinant mouse FCGR4 consists of 214 amino acids and has a predicted molecular mass of 24.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 35-40 kDa due to glycosylation.