FCGR2B Protein (C-His, Human)
Product Specifications
Abbreviation
FCGR2B
Synonyms
CD32, CD32B, FCG2, FcgammaRIIb, FCGR2, FCGR2C, FcGRIIB, FcRII-c, IGFR2
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Loaded Recombinant Human CD32B/Fcgr2b Protein, His Tag (Cat. No. 10259-H08H) on His1K Biosensor, can bind Anti-CD20 (Research Grade Rituximab Biosimilar) with an affinity constant of 1.13 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Anti-CD20 (Research Grade Rituximab Biosimilar) captured on Protein A chip can bind Recombinant Human CD32B/Fcgr2b Protein, His Tag (Cat. No. 10259-H08H) with an affinity constant of 0.7827 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Recombinant Human CD32b / FCGR2B Protein (His Tag) (Cat: 10259-H08H) captured on CM5 chip via Anti-His antibody, can bind Anti-CD20 (Research Grade Rituximab Biosimilar) with an affinity constant of 12.130 μM as determined in an SPR assay (Biacore T200) (QC tested) .
Function
Ala46-Pro217
Molecular Weight
The recombinant mature human CD32b consists of 183 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 20.8 kDa. By SDS-PAGE under reducing conditions, it migrates as an approximately 25-30 kDa protein due to glycosylation.
Species
Human
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