FCGR2B Protein (C-His, Human)

1.Immobilized Human FcγRIIB/C / CD32b/c recombinant protein (Cat. 10259-H08C) can bind biotinylated human IgG1, The EC50 is 60-180 ng/mL. 2.Loaded Human FcγRIIB/C / CD32b/c recombinant protein (Cat. 10259-H08C) on His1K Biosensor, can bind Rituximab (IgG1) with an affinity constant of 1.5 μM as determined in a BLI assay (Sartorius Octet Red384) . 3.Captured IgG1 on proteinA Chip can bind Human FcγRIIB/C / CD32b/c recombinant protein (Cat. 10259-H08C) with an affinity constant of 2.9 μM as determined in an SPR assay (Biacore T200) . 4.Captured Human CD32b/c recombinant protein (10259-H08C) on Anti-His Chip can bind IgG1 Fc Protein, His (Cat. 10702-HNAC) with an affinity constant of 37.5 μM as determined in an SPR assay (Biacore T200) .

The secreted recombinant human CD32b consists of 183 amino acids and predicts a molecular mass of 20.8 KDa. The apparent molecular mass of the protein is approximately 22-29 KDa in SDS-PAGE under reducing conditions due to different glycosylation.