FCGR2A Protein (C-AVI & His, Human)

Product Specifications

Abbreviation

FCGR2A

Synonyms

CD32, CD32A, CDw32, Fc-gamma-RIIa, FCG2, FcgammaRIIa, FcGR, FCGR2, FCGR2A1, IGFR2

Host

HEK 293

Tag

C-AVI & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE, ≥ 95 % as determined by SEC-HPLC, ≥ 95% as determined by SEC-MALS (Routinely tested) .

Bioactivity

1. Loaded Recombinant Human CD32a/FCGR2A Protein (H167, His&AVI Tag), Biotinylated (Cat: 10374-H27H1-B) on SA Biosensor, can bind anti-CD20 (Research grade Rituximab Biosimilar) with an affinity constant of 0.53 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 2. Recombinant Human CD32a/FCGR2A Protein (H167, His&AVI Tag), Biotinylated (Cat: 10374-H27H1-B) captured on SA chip, can bind anti-CD20 (Research grade Rituximab Biosimilar) with an affinity constant of 0.568 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Function

Met1-Met210

Molecular Weight

The recombinant human CD32a consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.7 KDa. It migrates as an approximately 33 KDa band due to glycosylation by SDS-PAGE under reducing conditions. The molecular weight of this protein is around 20-38 kDa verified by SEC-MALS (Routinely tested) .