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FCGR1 Protein (C-His, Human)

Product Specifications

Abbreviation

FCGR1

Host

CHO

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Anti-CD20 (Research Grade Rituximab Biosimilar) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD64 / FCGR1A Protein (ECD, His Tag) (Cat:10256-H08S), the EC50 0.7-2.1 ng/mL (QC tested) . 2.Loaded Herceptin on ProA Biosensor, can bind Recombinant Human CD64/FCGR1 Protein, His Tag (Cat. No. 10256-H08S) with an affinity constant of 1.73 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Recombinant Human CD64/FCGR1 Protein, His Tag (Cat. No. 10256-H08S) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 11.1 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Anti-CD20 (Research Grade Rituximab Biosimilar) captured on Protein A chip can bind Recombinant Human CD64/FCGR1 Protein, His Tag (Cat. No. 10256-H08S) with an affinity constant of 69.3 pM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 5.Recombinant Human CD64 / FCGR1A Protein (ECD, His Tag) (Cat: 10256-H08S) captured on CM5 chip via Anti-His antibody, can bind anti-ERBB2 (Research grade trastuz*mab biosimilar) with an affinity constant of 2.250 nM as determined in an SPR assay (Biacore 8K) (QC tested) .

Function

Met1-Pro288

Molecular Weight

The recombinant human FCGR1A consists of 284 amino acids and predicts a molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant human FCGR1A is approximately 45-60 kDa due to glycosylation. The molecular weight of this protein is around 46 kDa verified by SEC-MALS (Routinely tested) .

Species

Human

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