FCGR1 Protein (C-His, Human)

1.Immobilized Anti-CD20 (Ro) Antibody (Rituximab) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD64 / FCGR1A Protein (His Tag) (Cat:10256-H08H), the EC50 0.6-2.0 ng/mL. 2.Captured Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 5.5 nM as determined in an SPR assay (Biacore T200) . 3.Captured Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 13.6 nM as determined in an SPR assay (Biacore T200) . 4.Loaded Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 8.5 nM as determined in a BLI assay (Sartorius Octet Red384) . 5.Loaded Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 4.7 nM as determined in a BLI assay (Sartorius Octet Red384) . 6.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) with an affinity constant of 0.38 nM as determined in a BLI assay (Sartorius Octet Red384) . 7.Loaded Recombinant Human CD64/FCGR1 Protein, His Tag (Cat. No. 10256-H08H) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 6.36 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 8.Loaded Herceptin on proA Biosensor, can bind Recombinant Human CD64/FCGR1 Protein, His Tag (Cat. No. 10256-H08H) with an affinity constant of 0.213 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human CD64A consists of 284 amino acids and has a predicted molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant human CD64A is approximately 45-60 kDa due to glycosylation. The molecular weight of this protein is around 38-52 kDa verified by SEC-MALS (Routinely tested) .