FCER1A Protein (C-His, Human)

1.Immobilized Recombinant Human FcERI / FCER1A Protein (ECD, His Tag) (Cat: 13193-H08H) at 2 μg/mL (100 μl/well) can bind Recombinant Human IgE-Fc Protein (Constant Domain 3&4, His Tag) (Cat: 29702-H08H), the EC50 is 10-30 ng/mL. 2.Loaded Recombinant Human Fc epsilon RI Protein, His Tag (Cat. No. 13193-H08H) on AR2G Biosensor, can bind Recombinant Human IgE Fc Protein, His Tag (Cat. No. 29702-H08H) with an affinity constant of 1.96 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human FCER1A consists of 191 amino acids and predicts a molecular mass of 22.5 KDa. As a result of glycosylation, it migrates as an approximately 42-48 KDa band in SDS-PAGE under reducing conditions.