FCER1A Protein (C-His, Human)
Product Specifications
Abbreviation
FCER1A
Synonyms
FCE1A, FcepsilonRIalpha, FcERI, FCERIA
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Immobilized Recombinant Human FcERI / FCER1A Protein (ECD, His Tag) (Cat: 13193-H08H) at 2 μg/mL (100 μl/well) can bind Recombinant Human IgE-Fc Protein (Constant Domain 3&4, His Tag) (Cat: 29702-H08H), the EC50 is 10-30 ng/mL. 2.Loaded Recombinant Human Fc epsilon RI Protein, His Tag (Cat. No. 13193-H08H) on AR2G Biosensor, can bind Recombinant Human IgE Fc Protein, His Tag (Cat. No. 29702-H08H) with an affinity constant of 1.96 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Met1-Gln205
Molecular Weight
The recombinant human FCER1A consists of 191 amino acids and predicts a molecular mass of 22.5 KDa. As a result of glycosylation, it migrates as an approximately 42-48 KDa band in SDS-PAGE under reducing conditions.
Species
Human
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