F3 Protein (C-His, Human)

Product Specifications

Abbreviation

Synonyms

CD142, TF, TFA

Host

HEK 293

Tag

C-His

Endotoxin

< 0.5 EU per mg protein.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catalog# ES011) .

Function

Met1-Glu251

Molecular Weight

The recombinant human F3 consists of 230 amino acids and has a predicted molecular mass of 26.2 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 31.9 kDa verified by SEC-MALS (Routinely tested) .