F3 Protein (C-His, Human)

Product Specifications

Abbreviation

Synonyms

CD142, TF, TFA

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catalog# ES011) . The AC50 is < 5 μg/ml (QC tested) . 2.Immobilized Recombinant Human Coagulation Factor III/Tissue Factor Protein (His Tag) (Cat: 13133-H08H) at 1 μg/mL (100 μL/well) can bind Anti-F3 (Research Grade Tisotumab Biosimilar), the EC50 is 0.9 ng/mL (Routinely tested) .

Function

Met1-Glu251

Molecular Weight

The recombinant human F3 consists of 230 amino acids and has a predicted molecular mass of 26.2 kDa. It migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.