ERBB3 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

ERBB3

Synonyms

C-erbB-3, c-erbB3, ErbB-3, erbB3-S, FERLK, HER3, LCCS2, MDA-BF-1, p180-ErbB3, p45-sErbB3, p85-sErbB3, VSCN1

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized HER3/ERBB3 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:10201-H02H) at 2 μg/mL (100 μL/well) can bind NRG1 Beta 1 Protein, Human, Recombinant, Biotinylated (EGF Domain, Avi&Fc Tag) (Cat:11609-H01H-B), the EC50 is 220-1200 ng/mL (QC tested) . 2.Immobilized Recombinant Human HER3 / ErbB3 Protein (ECD, Fc Tag) (Cat: 10201-H02H) at 2 μg/mL (100 μL/well) can bind Anti-HER3 (Research Grade Patritumab Biosimilar), the EC50 is 2-6 ng/mL (Rountinely tested) . 3.Immobilized Recombinant Human HER3 / ErbB3 Protein (ECD, Fc Tag) (Cat: 10201-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human NRG1-beta 1 Protein (ECD, Fc Tag) (Cat: 11609-H04H), the EC50 is 4.0 ng/mL (Routinely tested) .

Function

Met1-Thr643

Molecular Weight

The mature recombinant human ErbB3/Fc chimera is a disulfide-linked homodimeric protein after the removal of signal peptide. The monomer consists of 862 amino acids and has a calculated molecular mass of 95.4 kDa. As a result of glycosylation, the recombinant monomer migrates as an approximately 130-140 kDa protein in SDS-PAGE under reducing conditions.