EGFR Protein (C-His, Cynomolgus)

Product Specifications

Abbreviation

EGFR

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC

Bioactivity

1. Immobilized EGF Protein, Human, Recombinant (ECD, hFc Tag) (Cat:10605-H01H) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Cynomolgus, Recombinant (ECD, His Tag) (Cat:90285-C08H), the EC50 is 150-500 ng/mL. 2. Immobilized Anti-EGFR (MK) -IgG1 Antibody (Cetuximab) (Cat:68069-H001) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Cynomolgus, Recombinant (ECD, His Tag) (Cat:90285-C08H), the EC50 is 2-8 ng/mL.

Function

Met1-Ser645

Molecular Weight

The recombinant cynomolgus EGFR consists of 632 amino acids and predicts a molecular mass of 70.1 kDa. As a result of glycosylation, it migrates as an approximately 93.8 kDa band in SDS-PAGE under reducing conditions.