EGF Protein (Native, Human)

1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2 (Cat#10014-HNAE), VEGFA (Cat# 11066-HNAH), EGF (Cat#10605-HNAE) . Red arrows represent vascular organoids. Image taken at 10x magnification. (Routinely tested) 2.Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.2ng/ml. 3.Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with EGF (Cat # 10605-HNAE) (10 ng/mL, 30min) using Phospho-EGF Receptor (Tyr1068) rabbit monoclonal Antibody (Cat# 110463-R0027) at 1:2000 dilution (upper), or Anti-EGFR Antibody, Rabbit Polyclonal (Cat# 100372-T32) at 1:2000 dilution (lower) (Routinely tested) .

The recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.35 kDa as estimated in SDS-PAGE under reducing conditions.