CTSB Protein (C-His, Human)

Product Specifications

Abbreviation

CTSB

Synonyms

APPS, CPSB, KWE, RECEUP

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog # ES008) The specific activity is >2,500 pmoles/min/μg. (Activation description: The enzyme achieves its activity under buffer with DTT)

Function

Arg18-Ile339

Molecular Weight

The recombinant human CTSB existing as the proform consists of 332 amino acids and has a predicted molecular mass of 37.2 kDa. rhCTSB migrates with the molecular weight of 43 and 36 kDa as the proform and mature form rspectively in SDS-PAGE under reducing conditions.