CTLA4 Protein (C-His, Human)

1.Immobilized Recombinant Human CD86 / B7-2 Protein (Fc Tag) (Cat:10699-H02H) at 2 μg/ml (100 μl/well) can bind Recombinant Human CTLA-4 Protein (His Tag) (Cat:11159-H08H), the EC50 is 2.6-8 ng/mL (Routinely tested) . 2. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.05-0.3μg/mL when stimulated with 1 μg/mL Recombinant Human B7‐1/CD80 (Routinely tested) . 3.Captured Anti-CTLA4 (Research grade Ipilimumab biosimilar) on proA Chip can bind CTLA-4 (Cat.No.11159-H08H) with an affinity constant of 20.03nM as determined in a SPR assay (Biacore T200) (Routinely tested) . 4.Loaded Anti-CTLA4 (Research grade Ipilimumab biosimilar) on ProA Biosensor, can bind CTLA-4 Protein, Human, Recombinant, His Tag (Cat.No.11159-H08H) with an affinity constant of 5.15nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested) . 5.Loaded Recombinant Human B7-1 Protein, hFc Tag (Cat. No. 10698-H02H) on ProA Biosensor, can bind Recombinant Human CTLA-4 Protein, His Tag (Cat. No. 11159-H08H) with an affinity constant of 2.66nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 6.Loaded Recombinant Human CD86 Protein, hFc Tag (Cat. No. 10699-H02H) on ProA Biosensor, can bind Recombinant Human CTLA-4 Protein, His Tag (Cat. No. 11159-H08H) with an affinity constant of 5.34nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 7.Immobilized Recombinant Human CD80 / B7-1 Protein (ECD, Fc Tag) (Cat: 10698-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 / CD152 Protein (His Tag) (Cat: 11159-H08H), the EC50 is 0.1-2 ng/mL (QC tested) .

The recombinant human CTLA4 consists of 138 amino acids and has a predicted molecular mass of 15 kDa. As a result of glycosylation, the apparent molecular mass of human CTLA4 is approximately 22-27 kDa in SDS-PAGE under reducing conditions