CSF2 Protein (N-His, Human)

Product Specifications

Abbreviation

CSF2

Synonyms

CSF, GM-CSF, GMCSF

Host

HEK 293

Tag

N-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 92 % as determined by SDS-PAGE

Bioactivity

1.Immobilized Recombinant Human GM-CSF / CSF2 Protein (His Tag) (Cat: 10015-H07H) at 1 μg/mL (100 μL/well) can bind Recombinant Human CSF2RA / GM-CSFR / CD116 Protein (Fc Tag) (Cat: 10701-H02H), the EC50 is 3-10 ng/mL (QC tested) . 2.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.6 ng/mL.

Function

Ala18-Glu144

Molecular Weight

The recombinant human GM-CSF consists of 147 amino acids and has a predicted molecular mass of 16.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh GM-CSF is approximately 24-27 kDa due to glycosylation.