CSF1R Protein (C-His, Human)

1.Measured by its binding ability in a functional ELISA. Immobilized human CSF1R (Cat:10161-H08H) at 10 μg/mL (100 μl/well) can bind biotinylated human CSF-1 (Cat:11792-H08H), The EC50 of biotinylated human CSF-1 (Cat:11792-H08H) is 8 ng/mL. 2.Captured M-CSF/CSF1 Protein, Human (Cat.No.11792-H02H) on proA Chip can bind CSF1R (Cat.No.10161-H08H) with an affinity constant of 0.1468 μM as determined in a SPR assay (Biacore T200) (Routinely tested) . 3.Loaded Biotinylated Recombinant Human M-CSF/CSF1 Protein, His & AVI Tag (Cat. No. 11792-H49H-B) on SA Biosensor, can bind Recombinant Human M-CSFR/CSF1R Protein, hFc Tag (Cat. No. 10161-H02H) with an affinity constant of 9.870 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human M-CSFR contains 504 amino acids after the removal of signal peptide with a calculated molecular mass of 56 kDa. As a result of glycosylation, it migrates as an approximately 85-95 kDa protein in SDS-PAGE under reducing conditions.