CSF1 Protein (C-His, Human)

1.Measured in a cell proliferation assay using M‐NFS‐60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 2-10 ng/mL. 2.Immobilized Recombinant Human CSF1R / MCSF Receptor / CD115 Protein (Fc Tag) (Cat: 10161-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human/Cynomolgus M-CSF/CSF-1 Protein (His Tag), Biotinylated (Cat: 11792-H08H-B), the EC50 is 0.8-2.4 ng/mL. (Routinely tested) 3.Loaded Recombinant Human M-CSF/CSF1 Protein, His Tag, Amine-Labeled Biotinylated (Cat. No. 11792-H08H-B) on SA Biosensor, can bind Recombinant Human M-CSFR/CSF1R Protein (Cat. No. 10161-HNAH) with an affinity constant of 0.474 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human CSF1 consists of 169 amino acids and predictes a molecular mass of 19.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCSF1 is approximately 27-32 kDa band due to glycosylation.