CHIKV-E2 Protein (C-His-AVI, CHIKV)

Product Specifications

Abbreviation

CHIKV-E2

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant CHIKV (UVE/CHIKV/2024/RE/CNR_79903) E2 Protein (His & AVI Tag) (Cat: 41094-V49H) at 1 μg/mL (100 μL/well) can bind CHIKV (strain SL-CK1) E2 / Envelope 2 Antibody, Mouse MAb (Cat: 40440-MM02), the EC50 is 1.2-4 ng/mL (QC tested) . 2.Immobilized Chikungunya virus (CHIKV) (UVE/CHIKV/2024/RE/CNR_79903) E2 / Envelope 2 Protein (His & AVI Tag) (Cat: 41094-V49H) at 1 μg/mL (100 μL/well) can bind rabbit anti- CHIKV antibody, the EC50 is 1.6 ng/mL (Routinely tested) . 3.Loaded Chikungunya virus (CHIKV) (strain SL-CK1) E2 / Envelope 2 Antibody, Mouse MAb (Cat. No. 40440-MM20) on AMC Biosensor, can bind Chikungunya virus (CHIKV) (UVE/CHIKV/2024/RE/CNR_79903) E2 / Envelope 2 Protein (His & AVI Tag) (HPLC-verified) (Cat. No. 41094-V49H) with an affinity constant of 6.51 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Ser326-Gln666

Molecular Weight

The recombinant Chikungunya virus (CHIKV) (UVE/CHIKV/2024/RE/CNR_79903) E2 / Envelope 2 comprises 378 amino acids and has a predicted molecular mass of 42.38 kDa.It migrates as approximately 51.2 kDa band in SDS-PAGE under reducing conditions.

Species

CHIKV