CHIKV-E2 Protein (C-His-AVI, CHIKV)
Product Specifications
Abbreviation
CHIKV-E2
Host
HEK 293
Tag
C-His-AVI
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Bioactivity
1.Immobilized Recombinant CHIKV (UVE/CHIKV/2024/RE/CNR_79903) E2 Protein (His & AVI Tag) (Cat: 41094-V49H) at 1 μg/mL (100 μL/well) can bind CHIKV (strain SL-CK1) E2 / Envelope 2 Antibody, Mouse MAb (Cat: 40440-MM02), the EC50 is 1.2-4 ng/mL (QC tested) . 2.Immobilized Chikungunya virus (CHIKV) (UVE/CHIKV/2024/RE/CNR_79903) E2 / Envelope 2 Protein (His & AVI Tag) (Cat: 41094-V49H) at 1 μg/mL (100 μL/well) can bind rabbit anti- CHIKV antibody, the EC50 is 1.6 ng/mL (Routinely tested) . 3.Loaded Chikungunya virus (CHIKV) (strain SL-CK1) E2 / Envelope 2 Antibody, Mouse MAb (Cat. No. 40440-MM20) on AMC Biosensor, can bind Chikungunya virus (CHIKV) (UVE/CHIKV/2024/RE/CNR_79903) E2 / Envelope 2 Protein (His & AVI Tag) (HPLC-verified) (Cat. No. 41094-V49H) with an affinity constant of 6.51 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Ser326-Gln666
Molecular Weight
The recombinant Chikungunya virus (CHIKV) (UVE/CHIKV/2024/RE/CNR_79903) E2 / Envelope 2 comprises 378 amino acids and has a predicted molecular mass of 42.38 kDa.It migrates as approximately 51.2 kDa band in SDS-PAGE under reducing conditions.
Species
CHIKV
Curated Selection
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