CGA & TSHB Protein (C-His, Human)
Product Specifications
Abbreviation
CGA & TSHB
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS (Routinely tested) .
Bioactivity
1.Label Recombinant Human TSH alpha & beta Heterodimer Protein (His Tag) (Cat: CT144-H08H) with biotin. Immobilized Recombinant Human TSH Receptor/TSHR (His Tag) (Cat: 17299-H08B) at 2 μg/ml (100 μl/well) can bind biotinylated Recombinant Human TSH alpha & beta Heterodimer Protein, the EC50 is 150-450 ng/mL (Rountinely tested) . 2.Loaded Recombinant Human TSHR Protein (Fc Tag) on proA Biosensor, can bind Recombinant Human TSH alpha & beta Heterodimer Protein (His Tag) (Cat. No. CT144-H08H) with an affinity constant of 70.7 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Ala25-Ser116 & Phe21-Val138
Molecular Weight
The recombinant heterodimer of Human TSH alpha & beta comprises 221 (103+118) amino acids and has a calculated molecular mass of 25.15 (11.65+13.50) KDa. The apparent molecular mass of human IL12B & IL23A heterodimer is approximately 22.6 kDa and 17.7 kDa respectively in SDS-PAGE. The molecular weight of this protein is around 26.8 kDa verified by SEC-MALS (Routinely tested) .
Species
Human
Curated Selection
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