CFD Protein (C-His, Human)

Product Specifications

Abbreviation

CFD

Synonyms

ADIPSIN, ADN, DF, PFD

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-_x0002_Lys-ThioBenzyl ester (ZLysSBzl), in the presence of 5,5’Dithiobis (2nitrobenzoic acid) (DTNB) .The specific activity is > 70 pmoles/min/μg.

Function

Ile26-Ala253

Molecular Weight

The secreted recombinant human CFD consists of 239 amino acids and predicts a molecular mass of 25.8 kDa. As a result of glycosylation, rh CFD migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.