CD80 Protein (C-His, Human)

1.Immobilized B7-1 Protein, Human, Recombinant (His Tag) (Cat: 10698-H08H) at 2 μg/ml (100 μl/well) can bind CTLA-4 Protein, Human, Recombinant (His & hFc Tag) (Cat: 11159-H03H) . The EC50 of of CTLA-4 (His & hFc Tag) (Cat: 11159-H03H) is 35-80 ng/ml (Routinely tested) . 2.Loaded Recombinant Human CTLA4 / CD152 Protein (Fc Tag) (Cat: 11159-H02H6) on ProA Biosensor, can bind Recombinant Human CD80 / B7-1 Protein (His Tag) (Cat: 10698-H08H) with an affinity constant of 210 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Recombinant Human B7-1 Protein, His Tag (Cat. No. 10698-H08H) on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag (Cat. No. 11524-H02H) with an affinity constant of 0.46 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested) (Routinely tested) . 4.Immobilized Recombinant Human CD80 / B7-1 Protein (His Tag) (Cat: 10698-H08H) at 1 μg/mL (100 μL/well) can bind Anti-CD80 (Research Grade Galix*mab Biosimilar), the EC50 is 1.2 ng/mL (Routinely tested) . 5.Immobilized Recombinant Human CTLA4 / CD152 Protein (Fc Tag) (Cat: 11159-H02H6) at 10 μg/mL (100 μL/well) can bind Recombinant Human CD80 / B7-1 Protein (His Tag) (Cat: 10698-H08H), the EC50 is 0.2-1 μg/mL (QC tested) . 6.Recombinant Cynomolgus CD28 / TP44 Protein (Fc Tag, ECD) (Cat: 90182-C02H) immobilized on CM5 chip, can bind Recombinant Human CD80 / B7-1 Protein (His Tag) (Cat: 10698-H08H) with an affinity constant of 10.500 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The recombinant human B7-1 consists of 219 amino acids and has a predicted molecular mass of 25.4 kDa. By reduced SDS-PAGE, the apparent molecular mass of rh B7-1 is approximately 45-48 kDa due to glycosylation.